Accession Number : AD0164808
Title : Enzymatic Hydrolysis of Cellulose to Soluble Sugars.
Descriptive Note : Patent,
Corporate Author : DEPARTMENT OF THE ARMY WASHINGTON D C
Personal Author(s) : Mandels,Mary H. ; Kostick,John A.
Report Date : 09 OCT 1973
Pagination or Media Count : 3
Abstract : The invention relates to the enzymatic hydrolysis of cellulose to soluble sugars. A stable cellulase enzyme obtained from a culture of Trichoderma viride QM 9123 is combined with cellulose to form a suspension having at least 100 parts by weight of cellulose to each part by weight of crude enzyme protein. The cellulose-cellulase suspension is maintained at a pH of from 4.0 to 5.0 and the temperature is maintained within the range of from 25 to 50C, during hydrolysis. Under these conditions and with the continuous addition of fresh cellulose to maintain the above ratio of cellulose to cellulase, the cellulase enzyme is effectively immobilized by adsorption on unhydrolyzed cellulose, allowing the removal of the soluble sugars by simple separation procedures while retaining most of the otherwise soluble enzyme material.
Descriptors : *Patents, *Cellulose, *Hydrolysis, Hydrolases, Glucose, Enzymes, Adsorption, Substrates
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE