Accession Number : AD0202185

Title :   STUDIES ON ORGANIC ACID METABOLISM,

Descriptive Note : Annual progress rept. 1 Jan 54-31 Dec 57,

Corporate Author : ILLINOIS UNIV URBANA

Personal Author(s) : Gunsalus ,I. C. ; Chin,C. H.

Report Date : 16 JUL 1958

Pagination or Media Count : 7

Abstract : Lipoic acid metabolism: The acetyl and succinyl thio esters of civinyl dimercapto were prepared by chemical and enzymatic means. The oxidation reduction reactions of the disulfide-dimercapto groups in pyrimidine nucleotide-linked reactions were explored in the initial lipoic acid assay organiam, Streptococcus faecalis, and in Escherichia coli, Proteus vulgaris, and Leuconostoc mesenteroides. These systems were compared to the reactions of the high molecular complex from pig heart and pigeon breast muscle, which contain the pyruvate and keto glutarate oxidizing systems of these tissues. The E. coli system linked suitably to lactic dehydrogenase with the reduction of pyruvate to lactic, indicating a lower potential for the mercapto-disulfide couple. The studies appeared to indicate a bound form of lipoic acid to be the coenzyme, and suggested that an amide or possibly another nonpolar linkage was the active form. Acid activating steps in energy transfer were studied via partial elucidation of the mechanism of the succinic acid activation. Phosphoryl-CoA functioned as an intermediate in the activating process and in energy transfer. (Author)

Descriptors :   BIOSYNTHESIS, ENZYMES, METABOLISM

Distribution Statement : APPROVED FOR PUBLIC RELEASE