Accession Number : AD0248879

Title :   ORGANO-TIN TOXICOLOGY

Descriptive Note : Annual progress rept. 1 Dec 59-30 Nov 60

Corporate Author : MICHIGAN UNIV ANN ARBOR

Personal Author(s) : BRODY, THEODORE M.

Report Date : 30 NOV 1960

Pagination or Media Count : 1

Abstract : MITOCHONDRIAL SWELLING AS EVIDENCED BY A DECREASE IN OPTICAL DENSITYWAS ALWAYS OBTAINED WITH CONCENTRATIONS OF TRIETHYLTIN (TET) AS LOW AS 1 X 10-7M. THE SWELLING INDUCED BY TET WAS UNAFFECTED BY THE ADDITION OF ETHYLENEDIAMINE TETRACETATE (EDTA) INDICATING THAT THE SWELLING OBSERVED WAS NOT THE RESULT OF AN IMPURITY SUCH AS INORGANIC TIN. IT WAS ALSO DEMONSTRATED THAT TET INHIBITS THE ATP REVERSAL OF SWELLING INDUCED BY THYROXIN. TET-INDUCED SWELLING (10-7M TET) COULD BE REVERSED BY ATP ADDITION, LESS READILY BY ADP AND NOT AT ALL BY AMP. FURTHER, THE ADDITION OF MN ION TOGETHER WITH THE ATP SEEMED TO POTENTIATE THE REVERSAL. WITH THIS COMBINATION IT WAS POSSIBLE TO REVERSE TETINDUCED SWELLING WHEN TET WAS PRESENT IN CONCENTRATIONS AS HIGH AS 10-4M. WHEN 2.4 DINITROPHENOL (2.4 DNP) WAS ADDED TO THE MEDIUM PRIOR TO TET ADDITION, IT EFFECTIVELY PREVENTED THE USUAL SWELLING

Descriptors :   *ADRENAL GLANDS, *BIOCHEMISTRY, *BRAIN, *HEART, *TIN COMPOUNDS, AMIDES, EPINEPHRINE, ETHYL RADICALS, HISTOLOGICAL TECHNIQUES, LIVER, ORGANIC COMPOUNDS, SEROTONIN.

Distribution Statement : APPROVED FOR PUBLIC RELEASE