Accession Number : AD0256396

Title :   THE EFFECT OF SOME KREBS CYCLE INHIBITORS AND INTERMEDIATES ON SHIGELLA INFECTIONS IN MICE

Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD

Personal Author(s) : FLOYD,THOMAS M. ; CLARK,ROBERTSON B.

Report Date : 04 NOV 1960

Pagination or Media Count : 1

Abstract : Recent reports have indicated that injection of substances inhibitory to enzymes of the tricarboxylic acid cycle, or of intermediate products of this cycle, would decrease survival time of mice challenged with a variety of organisms. Groups of mice were inoculated rectally with Shigella flexneri 3 organisms and then injected parenterally with one of the following: disodium malonate, sodium arsenite, trisodium citrate, disodium succinate. Control groups were given either organisms alone, tricarboxylic acid (TCA) cycle inhibitors or intermediates alone, or these compounds heat-killed organisms. Within 72 hours S. flexneri 3 organisms alone killed 4 (5.0%) of 80 mice. In combination with malonate they killed 15 (75%) of 20, with citrate 10 (50%) of 20, with succinate 8 (40%) of 20, and with arsenite, 8 (40%) of 20. There were no deaths among 80 mice inoculated with heat-killed organisms alone. Among those that received heatkilled organisms plus inhibitors or intermediate, 3 (15%) of 20 injected with malonate died, 6 (30%) of 20 with citrate, 0 of 20 with succinate, and 2 (20%) of 10 with arsenite. There was no evidence of diarrhea in surviving animals. Shigella were recovered less often from mice injected with malonate, citrate or succinate and more often from those that received arsenite than from controls inoculated with organisms alone. (Author)

Descriptors :   *DISEASES, *ENZYMES, *METABOLISM, *SHIGELLA, ARSENIC COMPOUNDS, CITRATES, INHIBITION, INJECTION, INTESTINES, MALONIC ACID, SUCCINATES, SURVIVAL(PERSONNEL)

Distribution Statement : APPROVED FOR PUBLIC RELEASE