Accession Number : AD0257295

Title :   RADIOPROTECTIVE AGENTS. ANALYSIS OF THE POTENTIAL ANTIRADIATION EFFECT OF NUCLEIC ACIDS OR THEIR PRECURSORS

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : ROUNDS,DONALD E.

Report Date : APR 1961

Pagination or Media Count : 1

Abstract : A total of nine compounds, including nucleic acids, purines, pyrimidines, and one nucleoside, were tested in vitro for activity as radioprotective agents. One agent which proved to be effective was yeast ribonucleic acid (RNA). Four others, which showed moderate potential, were calf thymus deoxyribonucleic acid (DNA), yeast DNA, orotic acid, and adenine. Guanine, xanthine, uracil, and deoxyadenosine had no protective effect in the test system. Yeast RNA was found to be relatively nontoxic to amnion cells over a 24-hr treatment period, but a chronic exposure of the cells to 0.5 mg./ml. for 6 days caused a decrease in the growth rate to about 52% of control cultures. Preirradiation treatment of seven different cell strains with 0.1 mg./ml. yeast RNA resulted in a widely variable protective response. Treated populations of nonmalignant origin showed more cells surviving 700 r gamma radiation than did untreated, irradiated control cultures. Malignant cellsincluding KB, HeLa, and Detroit 6 strain of bone marrow-showed no protective response. (Author)

Descriptors :   *MEDICINE, *NUCLEIC ACIDS, *PURINES, *PYRIMIDINES, *RADIATION EFFECTS, *RADIATION INJURIES, ADENINE, CONTROL, COUNTERMEASURES, DRUGS, GAMMA RAYS, NUCLEOSIDES, RADIOPROTECTIVE AGENTS

Distribution Statement : APPROVED FOR PUBLIC RELEASE