Accession Number : AD0262144

Title :   THE CONTENT OF ADENINE NUCLEOTIDES AND CREATINE PHOSPHATE IN BRAIN. I. NORMAL AND ANESTHETIZED RATS. II. EFFECT OF CHLORPROMAZINE

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : WEINER,NORMAN ; HULS,HARRIETT N.

Report Date : MAY 1961

Pagination or Media Count : 1

Abstract : IN UNANESTHETIZED RATS KILLED BY IMMERSION IN LIQUID OXYGEN, LEVELS OF ADENOSINE MONOPHOSPHATE, ADENOSINE DIPHOSPHATE, ADENOSINE TRIPHOSPHATE, AND CREATINE PHOSPHATE IN BRAIN WERE 0.42, 0.66, 1.22, AND 2.02 MICROMOLES/GM., RESPECTIVELY.Adenosine triphosphate and creatine phosphate are incompletely extracted from brain by perchloric acid unless sufficiently large ratios of volume of acid to sample size are used. During the extraction of the frozen tissue, some breakdown of labile phosphate compounds is apparently occurring, in spite of efforts to avoid this. Decapitation before immersion of the severed head in liquid oxygen results in much lower levels of brain adenosine triphosphate and creatine phosphate and higher levels of adenosine monophosphate. Anesthesia with either ether or pentobarbital before immersion of the whole animal in liquid oxygen results in the recovery of higher brain levels of creatine phosphate but in no change in levels of adenine nucleotides as compared with control animals. The activities of adenosine triphosphatases and creatine phosphokinase in rat brain are sufficiently great to be capable of destroying in a few seconds all the adenosine triphosphate and creatine phosphate present. (Author)

Descriptors :   *BRAIN, *CHLORPROMAZINE, *NUCLEOTIDES, *PHOSPHATES, ADENINE, CHROMATOGRAPHIC ANALYSIS, DETERMINATION, ENZYMES, INHIBITION, ION EXCHANGE, LABORATORY ANIMALS, METABOLISM, OXIDOREDUCTASES, PHARMACOLOGY, RECOVERY

Distribution Statement : APPROVED FOR PUBLIC RELEASE