Accession Number : AD0266165

Title :   MICROBIOLOGIC STUDIES WITH OZONE MUTAGENESIS OF OZONE FOR ESCHERICHIA COLI

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : DAVIS,IRVING

Report Date : JUN 1961

Pagination or Media Count : 1

Abstract : This report is one of a series of tudies d signed to provide an un erstanding of the biologic activity of ozone at the cellular level. The possible role of ozone as a biologic mutagenic agent was undertaken. Ozone was produced by a Welsbach water-cooled corona discharge ozonator using dry, clean oxygen. Analyses for ozone in aqueous solutions were carried out spectrophotometrically. Cells of Escherichia coli were exposed to aliquots of ozonated-wa er stock solutions. Bacterial survivors and mutants were determined by recognized plating procedures. T HE MUTAGENESIS OF OZONE IN AQUEOUS SOLUTIONS ON E. coli was studied in two mutational systems. Mutation frequencies in the streptomycin-dependence method appear o increase with increasing concentrations of ozone and decreasing survival. In the phage-resistance me hod, the frequency of zero-point mutants showed a significant increase after exposure of the cells to ozone. The importance of free radicals in biologic processes is discussed. It is suggested that a common pathway exists in the formation of these active radicals following irradiation, ozonization, or oxygen poisoning of biologic specimens. (Author)

Descriptors :   *ESCHERICHIA, *OZONE, BIOCHEMISTRY, MUTATIONS, OXYGEN, POISONING, SOLUTIONS(MIXTURES), SURVIVAL(PERSONNEL)

Distribution Statement : APPROVED FOR PUBLIC RELEASE