Accession Number : AD0266166

Title :   THE SEPARATION OF SERUM LIPIDS INTO FOUR MAJOR COMPONENTS BY SILICIC ACID CHROMATOGRAPHY

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : IKELS,KENNETH G.

Report Date : SEP 1961

Pagination or Media Count : 1

Abstract : A method is described for the separation of serum lipids by silicic acid chromatography into four major components (cholesterol ester, triglycerides, free cholesterol, and phospholipids). This method differs from others in current use in that it utilizes 1.2 gm. of silicic acid and a sample representing 0.8 ml. of serum. The separation of the lipid components can be accomplished in 4 to 5 hours. Essentially the method requires the preparation of small silicic acid columns. Introduction of a serum sample to the acid column results in elution of the four major lipid components wi h various percentage of ethyl ether in petroleum ether, methanol in chloroform, and absolute methanol. All effluents are quantitated colorimetrically with recoveries of 94 to 102 percent of known amounts of standards. (Author)

Descriptors :   *LIPIDS, CHROMATOGRAPHIC ANALYSIS, SEPARATION, SILICIC ACIDS

Distribution Statement : APPROVED FOR PUBLIC RELEASE