Accession Number : AD0266789

Title :   CONDUCT AN INVESTIGATION OF CIGUATERA POISON

Corporate Author : WORLD LIFE RESEARCH INST COLTON CALIF

Personal Author(s) : HALSTEAD,BRUCE W. ; HESSEL,DONALD W.

Report Date : 07 NOV 1961

Pagination or Media Count : 1

Abstract : Research on ciguatera toxin resulted in a satisfactory method for extracting the toxin from fish muscle. Partial purification was also accomplished by precipitation and silicic acid adsorption. The most precise fractionation of ciguatera toxin is accomplished on a silicic acid column developed in a stepwise fashion by other containing small percentages of methanol. It is felt that further experiments using silicicACID COLUMN CHROMATOGRAPHY WILL LEAD TO HIGHLY PURIFIED SAMPLES OF CIGUATERA TOXIN. Paper chromatography was examined using fourteen different solvent systems, but none proved satisfactory. A toxic fraction was found in the bluegreen alga, Lyngbya majascula from Palmyra Island which corresponds to the ciguatera fraction on the silicic acid column. This latter find may be a lead as to the source of the poison in nature, and may be of considerable CW-BW significance. (Author)

Descriptors :   *ALGAE, *FISHES, *TOXICITY, CHROMATOGRAPHIC ANALYSIS, DISTILLATION, MUSCLES, PURIFICATION

Distribution Statement : APPROVED FOR PUBLIC RELEASE