Accession Number : AD0267903

Title :   The Far Ultraviolet Absorption Spectra of Polypeptide and Protein Solutions and Their Dependence on Conformation,

Corporate Author : HARVARD UNIV CAMBRIDGE MASS

Personal Author(s) : ROSENHECK,K. ; DOTY,P.

Report Date : DEC 1961

Pagination or Media Count : 1

Abstract : The absorption spectra of polypeptides and proteins in aquenous solution in the region 185-240 millimicron were investigated and various factors contributing to this absorption were analysed. In polypeptides in the alphahelical form a large hypochromic effect is observed as well as a new feature in the form of a shoulder at 205 millimicron, which is not present in the spectrum of the random coil conformation. The absorption spectrum associated with the beta-form was recorded, and is shown to differ from each of the other two. The hypochromic effect is also observed in proteins, and by applying a correction for the absorption of chromophoric side chains, it is possible to arrive at a value for the helical content of the protein, hich agrees moderately well with data derived from optical rotatory dispersion measurements. (Author)

Descriptors :   ABSORPTION, AMIDES, AMINO ACIDS, CARBOXYLIC ACIDS, HEMOGLOBIN, INFRARED SPECTROSCOPY, INSULIN, METHIONINE, MUSCLE PROTEINS, PEPTIDES, PHENYLALANINE, PROTEINS, RIBONUCLEASE, SOLUTIONS(MIXTURES), TRYPTOPHAN, TYROSINE, ULTRAVIOLET SPECTROPHOTOMETERS, ULTRAVIOLET SPECTROSCOPY

Distribution Statement : APPROVED FOR PUBLIC RELEASE