Accession Number : AD0271806

Title :   THE DETERMINATION OF ESTERIFIED FATTY ACIDS IN GLYCERIDES, CHOLESTEROL ESTERS AND PHOSPHATIDES

Corporate Author : NAVAL WAR COLL NEWPORT R I

Personal Author(s) : SKIDMORE,W.D. ; ENTENMAN,C.

Report Date : 11 JAN 1962

Pagination or Media Count : 1

Abstract : The hydroxamic acid reaction conditions for the determination of esterified fatty acids were modified to the extent that the variable factors involved were controlled so that the molar absorptivities per ester group for triglycerides, cholesteryl esters of long-chain fatty acids, and phosphatides were equivalent through 8 microequivalent ester. The amount of water present during the formation of hydroxamates was the most important single factor in obtaining equivalent color values with these 3 types of esters. The accuracy and precision of the method were well defined by showing that the optical density values for 5 different ester standards were on the identical straight line curve. Spectral curves between the wavelengths of 410 and 700 millimicron with standard carboxylic acid esters and Folch extracts of rat serum, rat liver, and human serum were qualitatively and quantitatively identical. A long chain cholesteryl ester must be used as one of the standard esters because of its solubility characteristics and water sensitivity. Cholesteryl acetate can not be used as a reliable representative in place of a long chain cholesteryl ester. (Author)

Descriptors :   *FATTY ACID ESTERS, *HYDROXAMIC ACIDS, ABSORPTION, BLOOD SERUM, CARBOXYLIC ACIDS, CHEMICAL ANALYSIS, CHEMICAL REACTIONS, CHOLESTERYL ESTERS, DETERMINATION, PHOSPHOLIPIDS, RATS, SPECTROPHOTOMETERS, STANDARDS, TEST METHODS, VOLUMETRIC ANALYSIS

Distribution Statement : APPROVED FOR PUBLIC RELEASE