Accession Number : AD0282258

Title :   BASIC STUDIES IN PERCUTANEOUS ABSORPTION.

Descriptive Note : Semi-annual rept. no. 3, Jan-Jun 62,

Corporate Author : LEVER BROS CO EDGEWATER N J

Personal Author(s) : Vinson ,Leonard J. ; Koehler,Walter R.

Report Date : JUN 1962

Pagination or Media Count : 137

Abstract : The essential fatty acid (EFA) deficiency syndrome was related to the initial changes in function and structure of the barrier region of rats fed an EFA-free test diet. Skin recovery was also studied when the diet was supplemented with EFA. Lipid analyses of normal and dificient skins employing gas liquid and thin layer chromatography are given in detail. A fundamental study relating chemical structure and skin penetration rates of alkylamines was made. Penetration of the test material through skin was monitored by employing monoamine oxidase in the skin penetration vessel (modified Warburg) as described in previous reports. Also reported are the effects of repeated topical exposure to common solvents on skin permeability. Preliminary observations on the ultrastructure of the epidermis of both normal and EFA-deficient rats are reported. Electron micrographs depicting structural differences are presented. Studies of isolated epidermal proteins from the point of view of developing skin models to gain further insight into the nature of the skin barrier continue. Skin characterization from a physicochemical point of view is under study. Data relating to water diffusion through skin as a function of temperature and relative humidity are reported. (Author)

Descriptors :   *FATTY ACIDS, *SKIN(ANATOMY), ABSORPTION, ALKYL RADICALS, AMIDES, DIFFUSION, ELECTRON MICROSCOPY, HUMIDITY, LABORATORY ANIMALS, LIPIDS, ORGANIC SOLVENTS, PENETRATION, PRIVATION, PROTEINS, RATS, TEMPERATURE, WATER

Distribution Statement : APPROVED FOR PUBLIC RELEASE