Accession Number : AD0286744

Title :   RESEARCH ON PROCEDURES FOR THE LOW-TEMPERATURE PRESERVATION OF BLOOD

Corporate Author : UNION CARBIDE CORP TONAWANDA N Y LINDE DIV

Personal Author(s) : RINFRET,A.P.

Report Date : 25 APR 1960

Pagination or Media Count : 1

Abstract : Blood can be rapi ly frozen and thawed in bulk with red cell recovery above 90%. Protective additives such as glycerol, glucose, lactose, or polyvinylpyrrolidone (PVP) must be combined with blood to obtain such recovery. Red cell recovery is a function of the rate at which heat is transferred to or from the blood specimen. The nature of the additive combined with blood influences the requirement for heat transfer to achieve high red cell recovery. Thus, an additive which penetrates the cell, such as glycerol, requires slow cooling (0.3 C per second) in an isotonic medium to obtain highest red cell recovery. Extracellular additives such as lactose (22%) and polyvinylpyrrolidone (10%) require faster cooling rates (30 C per second) to obtain high recovery. Anions affect red cell recovery in freez -thaw procedure (using lactose as protective additive) in a sequence indicating a Hofmeister s ries. An assay for red cell viability in rabbits was developed. Aluminum containers were designed which permit efficient heat transfer through blood volumes up to 600 ml. (Author)

Descriptors :   *BLOOD, *ERYTHROCYTES, ADDITIVES, ANALYSIS, CONTAINERS, DESIGN, ELECTROLYTES (PHYSIOLOGY), ENZYMES, FREEZING, HEAT TRANSFER, LABELED SUBSTANCES, LIQUEFIED GASES, LOW TEMPERATURE RESEARCH, HUMANS, NITROGEN, RABBITS, TEMPERATURE, VIABILITY

Distribution Statement : APPROVED FOR PUBLIC RELEASE