Accession Number : AD0286752

Title :   RESEARCH ON PROCEDURES FOR THE LOW-TEMPERATURE PRESERVATION OF BLOOD. IX. STUDY OF METHODS FOR THE DETERMINATION OF IN VIVO SURVIVAL OF PRESERVED RED CELLS

Corporate Author : UNION CARBIDE CORP TONAWANDA N Y LINDE DIV

Personal Author(s) : RINFRET,A.P.

Report Date : 01 AUG 1961

Pagination or Media Count : 1

Abstract : A new method which meets both theoretical and practical criteria for assaying red cell survival of frozen and thawed blood was developed. An injection of chromium-51 labeled autologous red cells is made into a test animal followed by sampling of the peripheral blood for specific red cell radioactivity. A subsequent injection of preserved red c lls, also labeled with c romium51 but at a 10-fold higher level of radioactivity, is made into the same animal followed again by sampling of the peripheral blood for specific re cell radioactivity. The degrees of dilution of the two injections of chromiu -51 are compared. A detailed procedure and the derivation of an equation for calculating red cell survival are included. Comparison of our own and the work of others with respect to red cell survival of preserved red cells in rabbits and man has indica t validity of using rabbits in testing blood preservation methods. (Author)

Descriptors :   *BLOOD, *ERYTHROCYTES, *LABELED SUBSTANCES, CHROMIUM COMPOUNDS, FREEZING, PHOSPHORUS COMPOUNDS, RABBITS, RADIOACTIVE ISOTOPES, VIABILITY

Distribution Statement : APPROVED FOR PUBLIC RELEASE