Accession Number : AD0287065

Title :   EFFECTS OF INTRAVASCULAR HEMOLYSIS ON THE SPECIFIC ACTIVITY OF IRON-CONTAINING FRACTIONS OF RAT BLOOD

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Leffingwell ,Thomas P. ; Melville,George S. , Jr. ; Riess,Robert W.

Report Date : MAR 1962

Pagination or Media Count : 7

Abstract : INTRAVASCULAR HEMOLYSIS WAS INDUCED BY INJECTING PHENYLHYDRAZINE.HCl into rats which had received an intracardial injection of Fe59Cl3 in physiologic saline 168 hours earlier. At 24 hours after the phenylhydrazine injection the rats were sacrificed by exsanguination. Both stable iron and radioiron were analyzed in the residual blood cells, intact serum, and iron-containing serum fractions. Data were treated statistically. Results indicate that, whereas the specific activity of one of the iron-containing serum fractions does not exhibit the linear functions which characterize several other components of whole blood, it may reflect a dynamic equilibrium between erythrogenesis and cell catabolism. The ability to make such a distinction would be of great value in work with irradiated animals, and in other erythrogenic dyscrasias. (Author)

Descriptors :   *BLOOD PROTEINS, *HYDRAZINES, *PHENYL RADICALS, BLOOD SERUM, ELECTROPHORESIS, ERYTHROCYTES, HEMATOLOGY, IRON

Distribution Statement : APPROVED FOR PUBLIC RELEASE