Accession Number : AD0287067

Title :   CHROMOSOMAL STUDIES ON IRRADIATED LEUKOCYTES IN VITRO

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : OHNUKI,Y. ; POMERAT,C.M.

Report Date : DEC 1961

Pagination or Media Count : 1

Abstract : The technic for cultivation of the human buffy coat with heparinized venous blood treated with phytohemagglutinin was described. By this method chromosome spreads were made from 6-day cultures, using the colchicine-hypotonic, sodium citrateacetic, orcein-squash sequence. The range of polyploidy was determined on 2 specimens from each of 3 individuals (2 males and 1 female). Detailed analyses and counts of human chromosomes indicated the predominant distribution to be euploid cells having 46 chromosomes. Idiogram studies showed the same basic pattern demonstrated by previous investigators. These data served as a basis for comparing results obtained from sister cultures irradiated with 400 r from a cobalt-60 source on the fourth day and fixed on the sixth day. There was a considerable increase, 6.3 percent, in the number of polyploid cells. Among these, tetraploid cells were the most frequent, being 5.4 percent. By chromosomal analyses, 56.6 percent of the cells were found to be damaged. Most injuries were due to chromosomal breakages, especially fragments and dicentrics. Endoreduplication was noted frequently. (Author)

Descriptors :   *LEUKOCYTES, *RADIATION EFFECTS, AGGLUTININS, CHROMOSOMES, COBALT, GAMMA RAYS, HEPARIN, X RAYS

Distribution Statement : APPROVED FOR PUBLIC RELEASE