Accession Number : AD0287193

Title :   INFRARED STUDIES OF HUMAN SALIVA. IDENTIFICATION OF A FACTOR IN HUMAN SALIVA PRODUCING AN INFRARED ABSORBANCE MAXIMUM AT 4.9 MICRONS

Corporate Author : NAVAL DENTAL RESEARCH INST GREAT LAKES ILL

Personal Author(s) : LAMBERTS,BURTON L. ; MEYER,THOMAS S. ; FUCHS,HELMUTH H.

Report Date : JUN 1962

Pagination or Media Count : 1

Abstract : An absorption maximum was observed at 4.9 microns in infrared spectra of human parotid saliva. The factor causing this absorbance was found to be a dialyzable anion which was identified as thiocyanate on the basis of its solubility in ethanol and acetone, reactivity with ferric alum or ferric nitrate, and heat stability. Thiocyanate was then determined in 16 parotid saliva samples by a spectrophotometric method, which involved formation of ferric thiocyanate. The thiocyanate concentration of each sample was also estimated from the 4.9-micron absorbance. The mean results by the two methods did not differ significantly. Some limitations in the quantitative determination of thiocyanate in saliva from the 4.9-micron absorbance are discussed. The thiocyanate was not noticeably associated with the presence or absence of dental caries. (Author)

Descriptors :   *MEDICAL RESEARCH, *SALIVA, DETERMINATION, INFRARED SPECTROPHOTOMETERS, HUMANS

Distribution Statement : APPROVED FOR PUBLIC RELEASE