Accession Number : AD0297141

Title :   GENETIC DETERMINANTS OF COLICIN PRODUCTION, PRINCIPALLY THEIR RELATION WITH BACTERIOPHAGES AND WITH THE F AGENT OF FERTILITY

Corporate Author : LEEDS AND NORTHRUP CO PHILADELPHIA PA

Personal Author(s) : FREDERICQ,PIERRE

Report Date : 30 NOV 1962

Pagination or Media Count : 1

Abstract : Linkage of the V and B colicinogenic factors from wild strain K260 with an F agent was studied. The linkage is preserved after one and even 2 passages in the K12 strain. Some of the recombinants which received the B colicinogenic factor alone are highly fertile. They are probably of the same type. The B colicinogenic factor can be transfered from Hfr K12 to E.coli B, though the recombination rate are much lower than when both parents are K12. The colicinogenic factor is transfered in close association with the str markers but the fertility of recombinants is not easy to determine. Among the colicinogenic recombinants some are definitely Hfr, others are F+ and some appear to be F-. The 2 strains K94 and K260, previously studied in crosses with K12, were used as male parents in crosses with E.coli B. Results with K94 are similar to those obtained previously, except that many colicinogenic recombinants appear to be F-. In the cross with K260, the results are very different according to the selection used. Most recombinants selected for transfer of try are rather stable heterozygotes carrying a duplication of the try region, to which is linked the F agent and the colicinogenic factors V and B. They are highly fertile and probably of the F' type. (Author)

Descriptors :   *BACTERIA, BACTERIOPHAGES, CHROMOSOMES, ESCHERICHIA, GENETICS, MUTATIONS, SELECTION, STREPTOMYCINS

Distribution Statement : APPROVED FOR PUBLIC RELEASE