Accession Number : AD0404735

Title :   CULTIVATION OF PASTEURELLA TULARENSIS ON A SIMPLE LIQUID MEDIUM

Corporate Author : JOINT PUBLICATIONS RESEARCH SERVICE ARLINGTON VA

Personal Author(s) : Lukas, Bohumir

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Report Date : 14 MAR 1963

Pagination or Media Count : 12

Abstract : The possibility of cultivating Pasteurella tularensis on a simple medium, containing bacto peptone (Difco or Organofarma), glucose and NaCl, was confirmed. Growth on this medium depended on the size of the inocculum and the quantity of peptone contained in the medium. Beginning with a certain concentration of both peptones tried, an inhibition of growth was ob served (Organofarma - 1%, Difco - 2%). The Organofarma peptone had inferior growth proper ties than bactopeptone Difco. The cultivation conditions were favorably influenced by an addi tion of sodium thioglycollate (in the optimum proportion to the concentrat peptone) and particularly by an addition of 2-5% difibrinated rabbit blood. Enrichment of the medium with the above constituents intensified growth and permitted to reduce the inocculum on both peptones even to single microbes. A simple liquid medium was prepared - TKGL (sodium thioglycollate, blood, glucose and liquid). The qualities of this medium for cultivation and isolation were tested against the solid media. The liquid medium was found very good in these experiments.

Descriptors :   *CULTURE MEDIA, *FRANCISCELLA TULARENSIS, *PASTEURELLA, TISSUES(BIOLOGY), LIQUIDS, PEPTONES, TRANSLATIONS, CZECHOSLOVAKIA, BLOOD, LABORATORY ANIMALS

Subject Categories : MICROBIOLOGY

Distribution Statement : APPROVED FOR PUBLIC RELEASE

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