Accession Number : AD0407351

Title :   PURIFICATION OF STREPTOKINASE AND HUMAN PLASMIN AND THEIR INTERACTION,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Blatt, W.F. ; Segal, H. ; Gray, J.L.

Report Date : 17 JUN 1963

Pagination or Media Count : 17

Abstract : Procedures are described for the purification of streptokinase and human plasmin. Molecular weights of 42,600 for streptokinase and 88,800 for plasmin were determined from sedimentation and diffusion data. Equilibration of strep to kinase with plasmin produced an inhibition of caseinolytic activity and indicated a mole-mole interaction. The inhibitory activity was lost following acid treatment. These findings further support the concept of an interaction between human plasmin and streptokinase to form a com plex capable of converting plasminogen to plasmin. (Author)

Descriptors :   *PHOSPHORUS TRANSFERASES, *PLASMIN, HUMANS, BLOOD CHEMISTRY, BOVINES, ENZYMES, THROMBIN, PURIFICATION.

Distribution Statement : APPROVED FOR PUBLIC RELEASE