Accession Number : AD0410869

Title :   THE EFFECT OF ADENINE, INOSINE, INOSINE-ADENINE, AND ADENOSINE ON HUMAN ERYTHROCYTES DURING STORAGE.

Descriptive Note : Final technical rept., 1 Aug 62-31 July 63,

Corporate Author : SCRIPPS CLINIC AND RESEARCH FOUNDATION LA JOLLA CALIF

Personal Author(s) : Shafer,A.William ; Trombold,John S.

Report Date : 25 JUL 1963

Pagination or Media Count : 14

Abstract : Units of blood were divided into 5 aliquots and were stored at 4 C. in ACD without additive or supplemented with adenine, inosine, inosine adenine, or adenosine. Adenine and nucleoside were added in an amount of 0.75 and 15 micro moles per ml ACD-blood, respectively. After storage for 6 weeks, 24 hour post-transfusion survival of the erthrocytes was determined. Survival of the erthrocytes was significantly increased when the ACD was supplemented with any of the additives. There was, however, no significant difference in the effect of any one of the additives. The glycolytic intermediates of erythrocytes stored for the same period with the same additives were studied after separation on columns of ion-exchange resin. No adenosine triphosphate (ATP) or 2,3-diphosphoglycerate (DPG) remained in the blood stored in ACD alone. Supplementation of ACD with adenine resulted in no qualitative or quantitative change in the intermediates. ATP and DPG remained in the erythrocytes stored in ACD supplemented with inosine, inosine-adenine, or adenosine - con centration of ATP was highest with inosine adenine and of DPG was highest with adenosine. (Author)

Descriptors :   (*HEMATOLOGY, BLOOD TRANSFUSION), (*BLOOD, STORAGE), ERYTHROCYTES, ADENOSINE, PHOSPHORYLATION.

Distribution Statement : APPROVED FOR PUBLIC RELEASE