Accession Number : AD0412565

Title :   TRAION OF BACILLUS LICHENIFORMIS AND BACILLUS SUBTILIS BY EACH OF TWO PHAGES,

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Taylor, Martha J. ; Thorne, Curtis B.

Report Date : JUN 1963

Pagination or Media Count : 26

Abstract : A second transducing bacteriophage, designated SP-15, was isolated from the same soil sample culture filtrate that supplied the Bacillus sub tilis transducing phage, SP-10, reported earlier from this laboratory. SP-10 and SP-15 differ serologically and in several other respects, but share the ability to propagate on B. subtilis W-23-Sr (streptomycin-resistant) and Bacillus licheniformis ATCC 9945A, and to mediate general transduction in either species when propagated homologously. Attempts to transduce between the species have failed. Sp-10 forms plaques readily on both W-23-Sr and 9945A; SP-15 forms minute plaques on W-23-Sr and has shown no evidence of any lytic activity on 9945A. Maximum recoveries of prototrophic colonies from mixtures of SP-10 with auxotrophs of either W-23-Sr or 9945A were obtained only when excess phage was neutralized by post-transduction treatment with specific phage antiserum. Such treatment was not neces sary for maximum recovery of transductants effected by SP-15. SP-15 propagated on W-23-Sr did not transduce B. subtilis 168 (indole), but transduced B. licheniformis more efficiently than SP-10. Neither phage transduced B. licheniformis as efficiently as it transduced B. subtilis..

Descriptors :   (*BACILLUS SUBTILIS, GENETICS), (*BACTERIA, GENETICS), BACTERIOPHAGES, INFEC, EFFECTIVENESS, STREPTOMYCINS, CULTURE, PROPAGATION, BIOASSAY, INHIBI, GROWTH.

Distribution Statement : APPROVED FOR PUBLIC RELEASE