Accession Number : AD0424254

Title :   BIOLOGICAL SIGNIFICANCE OF HIGH MOLECULAR WEIGHT POLYPEPTIDES.

Descriptive Note : Final technical rept., 1 Apr 62-30 June 63,

Corporate Author : WEIZMANN INST OF SCIENCE REHOVOTH (ISRAEL)

Personal Author(s) : Katchalski,Ephraim

Report Date : 30 JUN 1963

Pagination or Media Count : 21

Abstract : A tritium-labeled poly-L-lysine, has been synthesized. Experiments on the inactivation of coliphage T2 with an I131-labeled copolymer of lysine and tyrosine (molar residue ratio 30:1) have shown that the copolymer enters the phage during the inactivation process. The inactivated phage is still capable of injecting its DNA together with the labeled polypeptide into the host cells of Escherichia coli. New techniques for the preparation of water-insoluble enzyme derivatives have been worked out. Water-insoluble urease and ribonuclease derivatives have been prepared. The mode of action of water-insoluble ribonuclease on low and high molecular weight substrates has been investigated. Membranes possessing papain activity have been prepared with the aid of bifunctional reagents such as bisdiazobenzidine. (Author)

Descriptors :   (*PEPTIDES, MOLECULAR PROPERTIES), AMINO ACIDS, BIOASSAY, LABELED SUBSTANCES, ENZYMES, RIBONUCLEASE, BIOCHEMISTRY, SYNTHESIS, VIRUSES, ESCHERICHIA

Distribution Statement : APPROVED FOR PUBLIC RELEASE