Accession Number : AD0425465

Title :   THE MODE OF ACTION OF ANTIBIOTICS INVOLVING THE BACTERIAL CELL WALL.

Descriptive Note : Annual progress rept., 1 July 62-30 June 63,

Corporate Author : HOOD COLL FREDERICK MD

Personal Author(s) : Ratney,Ronald S.

Report Date : 13 DEC 1963

Pagination or Media Count : 10

Abstract : It is nown that many antibiotics prevent bacterial growth by inhibiting the biosynthesis of bacterial cell walls. Thus, bacteria grown in the presence of penicillin form spherical protoplasts which are apparently whole cells without a rigid cell wall. This study has been aimed at elucidating the chemical structure of the cell wall of Bacillus anthracis and the relation of this structure to virulence and antibiotic action. The C-terminal amino acid of the peptide component of the cell walls of B. anthracis (Weybridge strain) has been shown to be alanine by hydrazinolysis methods on a micro scale. Micro and ultramicro scale methods for peptide analysis by hydrazinolysis and fluorodinitroaniline labelling have been developed using thin layer chromatography as an auxiliary tool. (Author)

Descriptors :   (*BACTERIA, CHEMICAL PROPERTIES), (*BACILLUS ANTHRACIS, ANTIBIOTICS), BIOSYNTHESIS, PENICILLINS, AMINO ACIDS, GROWTH(PHYSIOLOGY), INHIBITION, BIOLOGICAL STAINS

Distribution Statement : APPROVED FOR PUBLIC RELEASE