Accession Number : AD0429777

Title :   STUDIES ON THE CHANGE OF THE CONCENTRATIONS OF INTERMEDIATES DURING PHOTOSYNTHESIS OF CHLORELLA AND ISOLATED CHLOROPLASTS.

Descriptive Note : Final rept. no. 1,

Corporate Author : TECHNISCHE HOCHSCHULE MUNICH (WEST GERMANY)

Personal Author(s) : Kandler,O.

Report Date : 01 MAY 1963

Pagination or Media Count : 27

Abstract : The chages in the concentrations of intermediates and the distribution of C(14) after photosynthesis in C(14)02 by Chlorella and isolated chloroplasts was measured under different conditions of photosynthesis and under the effect of metabolic inhibitors. The following results were obtained. (1) The changes in the concentrations of intermediates in Chlorella during the transition from light to dark and vice versa are independent of CO2, but dependent on anaerobiosis. (2) In Chlorella photosynthesis is inhibited even by a concentration of monoiodoacetic acid which is 100 times lower than that inhibiting respiration. The beginning of inhibition is indicated by an accumulation of labeled PGA. (3) When Chlorella is illuminated in the presence of KCN, the radioactive PGA formed during a preceding period of photosynthesis in C(14)02, is still reduced to sugar without a change in the total quantity of PGA in the cell. (4) Arsenate and DNP do not lead to an accumulation of PGA, but do inhibit the formation of RuDP. (5) The distribution of radioactivity on the paper chromatograms showed that ATP and ADP are the first labeled compounds under the two conditions. (Author)

Descriptors :   (*PHOTOSYNTHESIS, TRACER STUDIES), (*ALGAE, PHOTOSYNTHESIS), CARBON DIOXIDE, CARBON COMPOUNDS, ILLUMINATION, LABELED SUBSTANCES, ADENOSINE PHOSPHATES, METABOLISM, CYANIDES

Distribution Statement : APPROVED FOR PUBLIC RELEASE