Accession Number : AD0456244

Title :   THE METABOLISM OF PELARGONATE-1-C14 BY WHEAT STEM RUST UREDOSPORES,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB

Personal Author(s) : Suryanarayanan,S. ; McConnell,W. B.

Report Date : 08 MAY 1964

Pagination or Media Count : 1

Abstract : Uredospores of Puccinia graminis were incubated in phosphate buffer (pH 6.2) containing pelargonic acid-1-C14. After 3 hours 97.5% of the tracer was assimilated. Fifty-five percent of this was released as C14O2 and 36.2% was incorporated into the spores. About one-half of the carbon-14 in the spores was soluble in ethanol and water, whereas nearly a third was ether extractable. The amino acid and carbohydrate fractions contained about equal amounts of carbon-14 and together accounted for twothirds of the radioactivity in the ethanol-water extract. The organic acids were also radioactive. Glutamic acid, gamma-aminobutyric acid, aspartic acid, and alanine were the most highly labelled amino acids. Fifty-three percent of the radioactivity in glutamic acid was found in carbon 1 and 46% in carbon 5. This distribution suggests beta-oxidation of perlargonic acid to acetyl CoA and extensive utilization of the latter by means of the glyoxylate cycle. (Author)

Descriptors :   (*FATTY ACIDS, METABOLISM), (*PUCCINIA, PHYSIOLOGY), TRACER STUDIES, RADIOACTIVE ISOTOPES, SPARES, BIOCHEMISTRY, AMINO ACIDS, CARBON COMPOUNDS, DISTRIBUTION, GLUTAMIC ACID, LABELED SUBSTANCES

Distribution Statement : APPROVED FOR PUBLIC RELEASE