Accession Number : AD0461500

Title :   TOXIN PRODUCTION BY CLOSTRIDIUM TETANI IN BIPHASIC LIQUID CULTURE,

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Puziss, Milton ; Heden, Carl-Goran

Report Date : APR 1965

Pagination or Media Count : 18

Abstract : An exploratory study is described on the use of a biphasic culture technique for production of toxin by C. tetani. The biphasic system used was a combination of dextran and polyethylene glycol (PEG) in the presence of a protein-free medium. The system supported food growth and gave a favorable phase ratio (top/bottom) of 15:1. The majority of the bacterial cells were in the bottom dextran phase, while the greater part of the toxin was in the upper PEG-medium phase. Toxin yields from a typical experiment were 10 to the 9th power mouse intraperitoneal minimum lethal doses per milliliter of upper phase filtrate. The biphasic culture system was also adaptable to continuous culture, with approximately similar toxin levels found in the upper phase. The appearance of the clostridial cells in older cultures suggested the possibility of protoplast formation, perhaps stabilized by the polymers present. In vitro toxin titration (Lf) was inhibited by the presence of the polymers. Bacillus anthracis also grew well in the biphasic system and elaborated protective antigen, primarily into the upper phase. (Author)

Descriptors :   (*CLOSTRIDIUM TETANI, TOXINS AND ANTITOXINS), CULTURE MEDIA, LIQUIDS, DEXTRAN, GLYCOLS, GROWTH(PHYSIOLOGY), PRODUCTION.

Distribution Statement : APPROVED FOR PUBLIC RELEASE