Accession Number : AD0474468

Title :   OBSERVATIONS ON NEWBORN RAT DORSAL ROOT GANGLIA IN VITRO FOLLOWING GAMMA IRRADIATION.

Descriptive Note : Rept. for 31 Mar 64-31 Mar 65,

Corporate Author : PASADENA FOUNDATION FOR MEDICAL RESEARCH CA

Personal Author(s) : Pomerat, C. M. ; Rounds, D. E. ; Raiborn, C. W., Jr. ; Pollard, T. D.

Report Date : NOV 1965

Pagination or Media Count : 8

Abstract : Newborn rats were exposed to gamma radiation at dosages ranging from 500 to 100,000 R. Unirradiated litter mates served as controls. Dorsal root ganglia were immediately explanted into Rose chambers and maintained for periods up to 21 days. Active growth, cone migration was observed from regenerating neurons 18 days after receiving 60,000 R. Neuronal nuclear rotation was recorded 25 days after 18,000 R. Schwann cells were observed to undergo rhythmic pulsations every 5 to 7 minutes. Radiation with either 6,000 R or 20,000 R produced no significant change in this contraction frequency. Some surviving Schwann and mesenchymal cells showed multinucleation, cytoplasmic vacuolation, and cellular enlargement following radiation. Hypertrophy was considered to be derived partially from multinucleation or altered membrane permeability. Treatment of unirradiated cultures with 5-fluorouracil resulted in Schwann-cell swelling within 3 days. By analogy, radiation may produce enlargement through inhibition of DNA replication during continuous RNA and protein synthesis. (Author)

Descriptors :   (*RADIATION EFFECTS, NERVE CELLS), CENTRAL NERVOUS SYSTEM, GANGLIA, RATS, RADIATION DOSAGE, TISSUE CULTURE CELLS, SENSITIVITY, RESISTANCE(BIOLOGY), IN VITRO ANALYSIS, GAMMA RAYS, CYTOLOGY, PROTEINS, BIOSYNTHESIS, PHYSIOLOGY.

Subject Categories : Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE