Accession Number : AD0474471

Title :   ASCORBIC ACID PROTECTION AGAINST INACTIVATION OF LYSOZYME AND ALDOLASE BY IONIZING RADIATION.

Corporate Author : ALBERT EINSTEIN MEDICAL CENTER PHILADELPHIA PA RADIATION RESEARCH LAB

Personal Author(s) : Shapiro, Bernard ; Kollmann, George ; Asnen, Joel N.

Report Date : NOV 1965

Pagination or Media Count : 0

Abstract : Solutions of lysozyme and aldolase (1% and 0.5%) were irradiated with Co60 gamma rays in the presence of different concentrations of ascorbic acid. This agent protected the enzymes even at 0.0005 M concentration. The protection was not quite as good as that obtained with GED when compared mole for mole, but was better than that obtained with dehydroascorbic acid. Radiation chemistry studies with C14-labeled ascorbic acid in enzyme solutions showed oxidation of ascorbic acid and binding to the enzymes. These results are interpreted in terms of mechanism of protective action. Comprehensive testing of ascorbic acid as a radiation-protective agent in animals is suggested. (Author)

Descriptors :   *ENZYMES), (*RADIATION EFFECTS, (*RADIOPROTECTIVE AGENTS, ASCORBIC ACID), RADIATION CHEMISTRY, MURAMIDASE, ALDEHYDE LYASES, BIOCHEMISTRY, EXPERIMENTAL DATA, RADIOBIOLOGY, GAMMA RAYS.

Subject Categories : Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE