Accession Number : AD0474601

Title :   FRACTIONATION OF BACTERIOPHAGE SP-10 DNA ON HYDROXYAPATITE COLUMNS,

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Mendelson, Neil H.

Report Date : OCT 1965

Pagination or Media Count : 12

Abstract : Bacteriophage SP-10 DNA may be fractionated by column chromatography on hydroxyapatite. Two components are readily isolated. One component consists of low molecular weight fragments. This component exhibits a typical single-strand thermal denaturation profile. The second component appears to be native double-strand DNA and exhibits a thermal denaturation profile indicative of double-stranded DNA. The original DNA preparation (prior to chromatography) exhibits a thermal denaturation profile which is the sum of the two individual profiles obtained from the column fractionated components. (Author)

Descriptors :   (*BACTERIOPHAGES, DEOXYRIBONUCLEIC ACIDS), DEOXYRIBONUCLEIC ACIDS, DISTILLATION, CHROMATOGRAPHIC ANALYSIS, BACILLUS SUBTILIS, MOLECULAR WEIGHT, MIXTURES, SEPARATION, TEST METHODS.

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE