Accession Number : AD0474601
Title : FRACTIONATION OF BACTERIOPHAGE SP-10 DNA ON HYDROXYAPATITE COLUMNS,
Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD
Personal Author(s) : Mendelson, Neil H.
Report Date : OCT 1965
Pagination or Media Count : 12
Abstract : Bacteriophage SP-10 DNA may be fractionated by column chromatography on hydroxyapatite. Two components are readily isolated. One component consists of low molecular weight fragments. This component exhibits a typical single-strand thermal denaturation profile. The second component appears to be native double-strand DNA and exhibits a thermal denaturation profile indicative of double-stranded DNA. The original DNA preparation (prior to chromatography) exhibits a thermal denaturation profile which is the sum of the two individual profiles obtained from the column fractionated components. (Author)
Descriptors : (*BACTERIOPHAGES, DEOXYRIBONUCLEIC ACIDS), DEOXYRIBONUCLEIC ACIDS, DISTILLATION, CHROMATOGRAPHIC ANALYSIS, BACILLUS SUBTILIS, MOLECULAR WEIGHT, MIXTURES, SEPARATION, TEST METHODS.
Subject Categories : Microbiology
Distribution Statement : APPROVED FOR PUBLIC RELEASE