Accession Number : AD0474985

Title :   HISTOCHEMICAL STUDIES ON THE DISTRIBUTION OF ENZYMES, ESPECIALLY OXIDASES AND PHOSPHATASES IN THE LIVING BODY.

Descriptive Note : Final rept. 29 Jul 64-28 Jul 65,

Corporate Author : KEIO UNIV TOKYO (JAPAN) DEPT OF ANATOMY

Personal Author(s) : Mitsui, Tadao

Report Date : 14 SEP 1965

Pagination or Media Count : 17

Abstract : A gigantic peroxidase granule was detected in the polymorphonuclear neutrophil leukocytes of the giant salamander, Megalobatrachus japonicus. The granule resembles the gigantism of the peroxidase granule that was recognized in human blood disease (Chediak and Higashi's disease). It is of interest that one of pathological characters of human leukocytes was found in an apparently healthy salamander. The pigmented leukocytes were found only in cold-blood animals such as reptiles and amphibia. These leukocytes could never be detected in man, mammals, birds and fishes. The pigmented leukocytes lack both peroxidase and PAS reacting substance suggesting that granules of the pigmented leukocytes are melanin injested in the blood forming organs. The peroxidase negative eosinophil leukocytes were detected in the liver tissue of Xenopus laevis Daudin. The lack of this enzyme in the eosinophil leukocyte suggests a profound philogenetical gap in comparative hematology. (Author)

Descriptors :   (*LEUKOCYTES, PEROXIDASES), ENZYMES, HISTOLOGY, AMPHIBIANS, CYTOCHEMISTRY, PIGMENTS, GRANULES, BLOOD CELLS.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE