Accession Number : AD0477587

Title :   REGULATION OF METABOLISM IN WORKING MUSCLE IN VIVO. I. CONCENTRATIONS OF SOME GLYCOLYTIC, TRICARBOXYLIC ACID CYCLE, AND AMINO ACID INTERMEDIATES IN INSECT FLIGHT MUSCLE DURING FLIGHT.

Descriptive Note : Rept. for 1962-1964,

Corporate Author : CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD

Personal Author(s) : Sacktor, Bertram ; Wormser-Shavit, Eva

Report Date : NOV 1965

Pagination or Media Count : 29

Abstract : The concentrations of 28 metabolites were measured concurrently in the flight muscle of the blowfly (Phormia regina) after flight periods ranging from 5 sec to 1 hr. Measurements were taken at flight initiation and during continuous flight. Coincident-change measurements identical to the 100-fold increase in glycolytic flux at the initiation of flight provide a unique system to determine the steps that control in vivo glycolysis in transition from a resting tissue to one performing strenuous work. The sites of regulation demonstrated in this system were (1) the phosphorolysis of fructose-6-P, (2) the cleavage of trehalose, and (3) the phosphorolysis of glycogen. Additional controls at alpha-glycero-P and proline oxidation are postulated. (Author)

Descriptors :   (*DIPTERA, PHYSIOLOGY), (*MUSCLES, BIOCHEMISTRY), GLYCOLYSIS, PHOSPHORYLATION, METABOLISM, IN VIVO ANALYSIS, AMINO ACIDS, TISSUE EXTRACTS, PYRUVATES, BLOOD ANALYSIS, THORAX, CARBOXYLIC ACIDS, POLYSACCHARIDES, FLIGHT.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE