Accession Number : AD0482510

Title :   INTERSPECIES TRANSFORMATION BETWEEN BACILLUS SUBTILIS AND BACILLUS LICHENIFORMIS,

Corporate Author : ARMY BIOLOGICAL DEFENSE RESEARCH CENTER FREDERICK MD

Personal Author(s) : Goldgerg, Ivan D. ; Gwinn, Darrel D. ; Thorne, Curtis B.

Report Date : APR 1966

Pagination or Media Count : 14

Abstract : Interspecies transformation was obtained between Bacillus subtilis and Bacillus licheniformis for the streptomycin resistance marker in both directions. A comparison of the reciprocal crosses indicates at least a 2-log difference in efficiency of transformation, depending on the direction of the cross. With B, licheniformis as the recipient and B, subtilis as the source of heterologous DNA, the number of interspecific transformants was 1/10,000 times the number of intraspecific transformants obtained with homologous DNA. When B, licheniformis DNA was used to transform B, subtilis number of interspecific transformants was only 1/10 to 1/100 times the number of intraspecific transformants. This work supports the existence of a conserved core of genetic information among the members of the genus Bacillus. (Author)

Descriptors :   *MUTATIONS), (*BACILLUS, BIOASSAY, GENETICS, BACILLUS SUBTILIS, STREPTOMYCINS, STREPTOCOCCUS, TRANSFORMATIONS.

Subject Categories : Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE