Accession Number : AD0484395

Title :   A HISTIDINE ACTIVE-CENTER DECAPEPTIDE FROM CHYMOTRYPSIN.

Descriptive Note : Technical rept. Dec 63-Dec 65,

Corporate Author : EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Personal Author(s) : Schaffer, Norwood K. ; Nordgren, Richard A. ; Orndorff, Bonny H. ; Balakir, Richard A. ; Michel, Harry O.

Report Date : JUL 1966

Pagination or Media Count : 21

Abstract : Chymotrypsin was inactivated with C14-labeled N-tosyl-L-phenyl-alanine chloromethyl ketone (TPCK), and the inactive enzyme was cleaved with performic acid and pepsin. A histidine active-center decapeptide was isolated by chromatography with Sephadex G-50, DEAE Sephadex A-25, Sephadex G-25, and Dowex 1-X2. Amino acid composition and end-group analysis showed its structure to be NH2-ala(ala, his-TPCK, CySO3H, thr, thr, gly, val, ser)-asp-COOH. (Author)

Descriptors :   *PEPTIDES), (*CHYMOTRYPSIN, AMINO ACIDS, LABELED SUBSTANCES, KETONES, CHROMATOGRAPHIC ANALYSIS, PEPSINS, FORMIC ACID.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE