Accession Number : AD0485846

Title :   A RAPID AND SIMPLE PHOSPHOLIPASE A ASSAY,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Kocholaty, Walter F.

Report Date : 30 DEC 1965

Pagination or Media Count : 7

Abstract : A simple and rapid method for the assay of phospholipase A was developed. As a substrate fresh egg yolk is used which is hydrolyzed by snake venom phospholipase A at a 10-20 x faster rate than pure lecithin. The released fatty acids, after extraction with appropriate solvents are titrated automatically. The performance of the electrodes in non-aqueous titrations is discussed. The described method will permit a great number of fast assays of phospholipase A, and the titration of fatty acids is free from subjective errors since it is carried out automatically. (Author)

Descriptors :   (*PHOSPHORIC MONOESTER HYDROLASES, MICROANALYSIS), TEST METHODS, VENOMS, HYDROLYSIS, EGGS, FATTY ACIDS, PURIFICATION, VOLUMETRIC ANALYSIS.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE