Accession Number : AD0600981

Title :   MECHANISM OF SNAKE VENOM ACTION.

Descriptive Note : Final technical rept., 1 Apr 62-31 May 64,

Corporate Author : IIT RESEARCH INST CHICAGO ILL

Personal Author(s) : Sumyk,George B. ; Kashin,Philip

Report Date : 31 MAY 1964

Pagination or Media Count : 94

Abstract : The in vivo distribution of radioiodine-labeled snake venoms and the preparation of a specific antiserum to the venom neurotoxin were the objectives of this research. Whole-animal autoradiographic studies showed that the labeled venoms were readily metabolized in mice and were excreted by various routes. Marked localization of cobra (Naja naja) and rattlesnake (Crotalus t. terrificus) venoms in the renal cortex was observed. The cottonmouth moccasin (Agkistrodon p. piscivorus) venom accumulated in the liver. Intravenously administered radioiodinated antivenin concentrated at the site of intramuscular venom injection. Antiserum to the neurotoxin of cobra venom was produced in rabbits and the gamma globulin fraction isolated. The antineurotoxin preparation protected mice against the whole cobra venom between 2 and 3 times better than commercial antivenin and neutralized up to 15-kg mouse LD (50) doses of the neurotoxin. Immunologic crossreactions among venoms of several cobra species were oberved. (Author)

Descriptors :   (*VENOMS, IMMUNE SERUMS), (*IMMUNE SERUMS, VENOMS), REPTILES, BITES AND STINGS, LABELED SUBSTANCES, AUTORADIOGRAPHY, GAMMA GLOBULIN, TRACER STUDIES, IMMUNITY, ANTIGEN ANTIBODY REACTIONS, TOXINS AND ANTITOXINS, NERVOUS SYSTEM, MICE

Distribution Statement : APPROVED FOR PUBLIC RELEASE