Accession Number : AD0612548

Title :   SOME BIOCHEMICAL AND PHYSICAL PROPERTIES OF THE HUMAN PERMEABILITY GLOBULINS,

Corporate Author : WALTER REED ARMY INST OF RESEARCH WASHINGTON D C

Personal Author(s) : Kagen,L. J.

Report Date : 02 FEB 1964

Pagination or Media Count : 9

Abstract : The permeability activity of globulin A is inhibited by C prime 1 esterase inhibitor, soy bean trypsin inhibitor, and di-isopropylfluorophosphate. TAMe portects against this DFP inhibition, and preparations of A contain SBTI inhibitable TAMe esterase. Preparations of A are also SBTI inhibitable kininogenases which liberate a smooth muscle contracting factor from both heated (63C, 1 hr.) and unheated plasma. A is estimated to have a sedimentation coefficient of approximately 11 S. It is concluded that globulin A is serum kallikrein. The permeability activity of globulin B is inhibited by C prime 1 esterase inhibitor, SBTI and DFP. TAMe protects against this DFP inhibition, and preparations of B contain SBTI inhibitable TAMe esterase. Preparations of globulin B are ineffective as kinin releasers when heated plasma (63C, 1 hr.) is used as the substrate. B is estimated to have a sedimentation coefficient of approximately 6 S. It is concluded that B is PF/dil. The inhibition of both permeability globulins by C prime 1 esterase inhibitor indicates that this inhibitor may be the permeability globulin inhibitor lacking in the disease, hereditary angioneurotic oedema. (Author)

Descriptors :   (*GLOBULINS, CHEMICAL PROPERTIES), PERMEABILITY, INHIBITION, CARBOXYLIC ESTER HYDROLASES, TRYPSIN, BLOOD SERUM, BLOOD PLASMA, ENZYMES, BIOASSAY, IMMUNITY

Distribution Statement : APPROVED FOR PUBLIC RELEASE