Accession Number : AD0614284

Title :   UNBALANCED GROWTH AND LATENT KILLING OF ESCHERICHIA COLI FOLLOWING EXPOSURE TO SULFUR MUSTARD.

Descriptive Note : Rept. for Feb 62-Mar 64,

Corporate Author : CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD

Personal Author(s) : Papirmeister,Bruno ; Davison,Claire L.

Report Date : FEB 1965

Pagination or Media Count : 49

Abstract : This report describes the effects of unbalanced metabolism on the survival of strains of Escherichia coli treated with sulfur mustard. Strains studied required thymine, arginine, and uracil (15T(-)A(-)U(-)) and thymine and phenylaniline (15T(-)PA(-)). Resistant bacteria incubated for 2 hr in a +T, -PA medium became progressively sensitive to sulfur mustard when transferred to a -T, -PA medium. Return to their original medium restored their original resistance. Resistant cells preincubated in +T, -AU showed higher survivals and were less susceptible to latent killing on post-treatment incubation in a -T, -AU medium. Controls in this medium remained immune to the induction of thymineless death, while mustard-treated cells showed small, dose-dependent, latent-killing effects. Pretreatment in thymidine media gave highest resistances and immunities to thymineless death. Resistant state was developed by subjecting mustard-treated cells to low levels of unbalanced metabolism. Unbalanced metabolism effects DNA and RNA stability. The monofunctional analog, beta-chloroethyl beta-hydroxyethyl sulfide, does not inhibit DNA synthesis nor promote its breakdown. Cross-alkylating reactions are effective for initiating unbalanced metabolism. (Author)

Descriptors :   (*ESCHERICHIA COLI, MUSTARD AGENTS), (*MUSTARD AGENTS, ESCHERICHIA COLI), METABOLISM, CULTURE MEDIA, DEGRADATION, IMMUNITY, GROWTH(PHYSIOLOGY), DEOXYRIBONUCLEIC ACIDS, RIBONUCLEIC ACIDS, RADIOACTIVE ISOTOPES, VIABILITY, INHIBITION, SYNTHESIS, STABILITY, NUCLEOSIDES

Distribution Statement : APPROVED FOR PUBLIC RELEASE