Accession Number : AD0614396

Title :   APPROXIMATE ENZYMIC METHOD OF MICROANALYSIS FOR IDENTIFYING RESIDUAL AMOUNTS OF ORGANOPHOSPHORUS INSECTICIDES,

Corporate Author : FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO

Personal Author(s) : Pokrovskii,A. A. ; Ponomareva,L. G.

Report Date : 30 MAR 1965

Pagination or Media Count : 17

Abstract : A simple, highly sensitive group method of detecting phosphorus-containing insecticides in various objects of the surroundings, based on their inherent anticholinesterase action, was developed. In order to realize this method a portable kit was constructed which contains a rack for the storage of ampouled reagents. The kit contains enough reagents for 100 analyses. The detection of insecticides may be performed under field conditions. A preliminary determination of insecticide contamination of products may be obtained in 10 - 20 min. The anticholinesterase effect of 6 of the most important insecticides was studied and calibration curves were constructed for their determination. The method (without preliminary activation) makes it possible to determine from 1 to 100 micrograms of insecticide per 1 ml. It was established that the anticholinesterase activity of chlorphos when dissolved in buffer solutions of pH 7.4 and under the action of plant extracts gradually increases. This is evidently explained by a conversion of the insecticide into a compound possessing high anticholinesterase activity. (Author)

Descriptors :   (*ORGANIC PHOSPHORUS COMPOUNDS, INSECTICIDES), (*MICROANALYSIS, ORGANIC PHOSPHORUS COMPOUNDS), (*INSECTICIDES, CHEMICAL ANALYSIS), ENZYMES, CHOLINESTERASE INHIBITORS, PORTABLE EQUIPMENT, CHEMICAL INDICATORS, CHOLINES, CHOLINESTERASE, USSR

Distribution Statement : APPROVED FOR PUBLIC RELEASE