Accession Number : AD0617590

Title :   SELECTIVE INACTIVATION OF FIBRIN STABILIZING FACTOR CONTAMINANT IN FIBRINOGEN,

Corporate Author : PRESBYTERIAN-ST LUKE'S HOSPITAL CHICAGO ILL

Personal Author(s) : Swigert,Stevenson ; koppel,J. L. ; Olwin,John H.

Report Date : 1962

Pagination or Media Count : 4

Abstract : The finding that mercaptide-forming agents inactivate Fibrin Stabilizing Factor (FSF) present in fluoresceinlabelled human or bovine fibrinogen preparations led to the development of a combined procedure in which both FSF inactivation and fibrinogen labelling with fluorescein are carried out simultaneously. This combined procedure makes possible the rapid and reproducible preparation of a labelled fibrinogen product suitable for use in an assay system for the quantitative measurement of FSF activity. It is the purpose of this paper to describe: (a) the procedure used for the preparation of such tagged, treated fibrinogen; (b) some of the characteristics of the latter; (c) its application to the assay of FSF activity.

Descriptors :   (*FIBRIN, BLOOD COAGULATION), (*FIBRINOGEN, QUANTITATIVE ANALYSIS), ENZYMES, BLOOD PROTEINS, STABILIZATION, LABELED SUBSTANCES, PREPARATION

Distribution Statement : APPROVED FOR PUBLIC RELEASE