Accession Number : AD0618254

Title :   IRON HEMATOXYLIN CHELATES. I. THE WELL STAINING BATH,

Corporate Author : CIVIL AEROMEDICAL INST OKLAHOMA CITY OKLA

Personal Author(s) : Berube,Gene R. ; Powers,Margaret M. ; Clark,George

Report Date : 1964

Pagination or Media Count : 10

Abstract : The procedure for the preparation of the staining solution for the Weil myelin sheath stain was systematically varied in respect to pH, concentration, time, temperature, and relative proportions of the ingredients. The results were explainable on the basis of the presence of a number of iron hematoxylin chelates in the staining bath. Compounds of the form of (Fe sub n Hem)(m+) are nuclear stains, those of the form of (FeHem sub n)(m=) are myelin sheath stains while the precipitate is probably (Fe sub n Hem sub y) sub x (m=). The following procedure for the stain is recommended. Mix equal portions of a 0.25% solution of ripened hematoxylin prepared from a 10% alcoholic solution and 1% ferric ammonium sulphate and use immediately. Preferably, the solutions should be at a temperature of about 5C and the staining done in the refrigerator, but room temperature may be used. Higher temperatures are contraindicated. Hematein should not be substituted for ripened hematoxylin; the resulting stains are too weak to be usable. The absorbance of hematein is no measure of the concentration of the component that stains myelin sheaths. Hematein apparently consists largely of a sparingly soluble highly colored inactive compound. (Author)

Descriptors :   (*BIOLOGICAL STAINS, PREPARATION), (*HISTOLOGICAL TECHNIQUES, BIOLOGICAL STAINS), NERVES, PH FACTOR, TEMPERATURE, TIME, IRON COMPOUNDS, COMPLEX COMPOUNDS

Distribution Statement : APPROVED FOR PUBLIC RELEASE