Accession Number : AD0618450

Title :   A SELF-LIMITING, HIGHLY SELECTIVE CHROMIUMHEMATOXYLIN STAIN,

Corporate Author : CIVIL AEROMEDICAL INST OKLAHOMA CITY OKLA

Personal Author(s) : Berube,Gene R. ; Powers,Margaret M. ; Clark,George

Report Date : 1965

Pagination or Media Count : 5

Abstract : A compound, which is probably a cationic chelate, can be isolated as a dry powder from a hematoxylinchrome alum lake. In aqueous acid solution this compound is an excellent nuclear stain which is extremely selective, very resistant to acids and alcohols, and self-limiting. Staining time may vary from 20 min to 16 hr without causing significant differences in staining intensity. To prepare the dry stain, dissolve 10 gm of hematoxylin, 10 gm of NaOH and 70 gm of chrome alum in 600 ml of distilled water, boil 20 min, cool and filter, allowing the filtrate to drop into 3.5 liters of absolute alcohol. Filter off the precipitate formed in the alcohol, and air dry it at room temperature. The staining solution is prepared by dissolving 3 gm of the dried precipitate in 100 ml of 3% HCl. (Author)

Descriptors :   (*BIOLOGICAL STAINS, PREPARATION), (*CHROMIUM COMPOUNDS, BIOLOGICAL STAINS), (*ORGANIC PIGMENTS, BIOLOGICAL STAINS), COMPLEX COMPOUNDS, NUCLEI(BIOLOGY), COLORING, HISTOLOGICAL TECHNIQUES, AMMONIUM COMPOUNDS, SULFATES

Distribution Statement : APPROVED FOR PUBLIC RELEASE