Accession Number : AD0620473

Title :   KINETIC STUDIES ON THE THERMAL DENATURATION OF DEOXYRIBONUCLEIC ACID WITH A HIGH-FREQUENCY HEATING METHOD,

Corporate Author : ARMED FORCES INST OF PATHOLOGY WASHINGTON D C

Personal Author(s) : Takashima,Shiro ; arnolds,Eugene A.

Report Date : 24 JUL 1964

Pagination or Media Count : 12

Abstract : The rate of heat denaturation of calf-thymus, salmonsperm and bacterial DNA's was studied with a highfrequency electric heating system. It was found that the rate of heat denaturation of DNA is rather slow, requiring more than 3 sec. The rate of denaturation is dependent on the guanine-cytosine content, and it is faster when the guanine-cytosine content is smaller. The rates are also faster below pH 5.0. Furthermore, the rate is faster in the presence of 8 M urea in the case of salmonsperm DNA but is not affected by urea in the case of calfthymus DNA. The relationship between the rate of unwinding and the length of DNA is also investigated but because of the possible presence of single-strand breaks, the results are complicated and no conclusion is reached concerning this problem. (Author)

Descriptors :   (*DEOXYRIBONUCLEIC ACIDS, HEATING), MOLECULAR STRUCTURE, INDUCTION HEATING, HIGH FREQUENCY, NUCLEOTIDES, UREA, DECOMPOSITION, PH FACTOR, REACTION KINETICS, GUANINE

Distribution Statement : APPROVED FOR PUBLIC RELEASE