Accession Number : AD0620629

Title :   ISOLATION OF COAGULANT AND ANTICOAGULANT PRINCIPLES FROM THE VENOM OF TRIMERESURUS GRAMINEUS,

Corporate Author : NATIONAL TAIWAN UNIV TAIPEI COLL OF MEDICINE

Personal Author(s) : Shiau,Shoei-yn ; Ouyang,Chaoho

Report Date : 1965

Pagination or Media Count : 9

Abstract : The venom of Trimeresurus gramineus was separated into five fractions, namely alpha, beta, gamma, delta, and epsilon by means of zone electrophoresis on starch with borate-NaCl buffer; pH 7.6, and ionic strength 0.062. Only the alpha-fraction migrated towards the anode. The beta-fraction remained at the position of application and the other three fractions migrated towards the cathode. The highest thrombin-like and TAMe-esterase activities were located next to the peak of the alpha-fraction on the anode side. Both activities were about seven times stronger than those of the crude venom. The proteolytic activity of the alpha-fraction was less than one half that of the crude venom. The beta-fraction showed only feeble thrombinlike, TAMe-esterase and proteolytic activities. The gamma-fraction was the principal portion with anticoagulant as well as proteolytic activities. The delta and epsilon-fractions had little effect on blood coagulation, although a moderate proteolytic activity was present in the delta-fraction. (Author)

Descriptors :   (*VENOMS, BLOOD COAGULATION), (*ANTICOAGULANTS, VENOMS), REPTILES, THROMBIN, PROTHROMBIN, FIBRINOGEN, ENZYMES, PEPTIDE HYDROLASES, CARBOXYLIC ESTER HYDROLASES, ELECTROPHORESIS, TAIWAN

Distribution Statement : APPROVED FOR PUBLIC RELEASE