Accession Number : AD0620977

Title :   ZONE ELECTROPHORESIS OF HUMAN PAROTID SALIVA IN ACRYLAMIDE GEL,

Corporate Author : NAVAL DENTAL RESEARCH INST GREAT LAKES ILL

Personal Author(s) : Meyer,T. S. ; Lamberts,B. L.

Report Date : MAR 1965

Pagination or Media Count : 5

Abstract : The report concerns an examination of acrylamide gel as another medium for the zone electrophoresis of parotid saliva proteins. Preliminary experiments with acrylamide-gel strips yielded protein patterns in which twenty or more fractions could be visually distinguished when the electrophoresis was carried out in 0.1 M tris-EDTAboric acid buffer at pH 9.0. Under the conditions of the experiment, more than half the staining components migrated anodically with generally better resolution than is evident for the cathodically-migrating components. Salivary amylase, a troublesome factor in the starch-gel electrophoresis of saliva proteins, does not attack acrylamide gel.

Descriptors :   (*SALIVA, ELECTROPHORESIS), (*ELECTROPHORESIS, SALIVA), PROTEINS, SEPARATION, GLYCOSIDE HYDROLASES, GELS, AMIDES

Distribution Statement : APPROVED FOR PUBLIC RELEASE