Accession Number : AD0621062

Title :   ELECTRON MICROSCOPY METHODS OF INVESTIGATION OF BIOLOGICAL OBJECTS,

Corporate Author : FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO

Personal Author(s) : BIRYUKOVA,V. I. ; Borovyagin,V. L. ; Gilev,V. P. ; Kiselev,N. A. ; Tikhonenko,A. S.

Report Date : 13 APR 1965

Pagination or Media Count : 250

Abstract : Basic methods of investigating biological objects are outlined. Special features of biological preparations which impart characteristic features to the process of preparing specimens are examined. Methods of contrasting with the help of shadowing, dyeing with salts of heavy metals, and also the method of 'negative' contrasting are examined in detail. Various means of preparing replicas are described. The questions of fixation and dehydration of objects and the methodology of preparing ultrathin sections are examined in detail. Some practical problems of working with the electron microscope and questions of photographic technique are outlined briefly. (Author)

Descriptors :   (*ELECTRON MICROSCOPY, BIOLOGY), ELECTRON MICROSCOPES, OPTICAL EQUIPMENT COMPONENTS, PHOTOGRAPHIC FILM, PHOTOGRAPHIC CONTRAST, BACTERIA, VIRUSES, TISSUES(BIOLOGY), HISTOLOGICAL TECHNIQUES, BIOLOGICAL STAINS, CALIBRATION, USSR

Distribution Statement : APPROVED FOR PUBLIC RELEASE