Accession Number : AD0621528

Title :   5-HYDROXYTRYPTAMINE RELEASE BY BRAIN SLICES.

Descriptive Note : Technical rept. for Sep 61-Jul 63,

Corporate Author : CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD

Personal Author(s) : Bowers,Malcolm B. ,Jr. ; Roodman,Stanford T. ; Filbert,Margaret G.

Report Date : JUN 1965

Pagination or Media Count : 18

Abstract : Brain slices from the rat were allowed to respire for 2 hr in a modified Tyrode's solution containing the monoamine oxidase inhibitor 1-phenyl-2-hydrazinopropane (alpha-methyl phenyl hydrazine, JB-516). The filtered Tyrode's solution was shown to contain 5-hydroxytryptamine (Serotonin, 5-HT) by both bioassay and fluorometric techniques. Preliminary evidence indicates that the amount of 5-HT in the filtrate is increased significantly by adding reserpine and JB-516 to the incubation medium. These findings suggest that a sensitive bioassay procedure, coupled with the use of brain slices. offers an approach for the study of factors affecting the storage and release of endogenous 5-HT. (Author)

Descriptors :   (*BRAIN, SEROTONIN), (*SEROTONIN, BIOASSAY), RATS, CENTRAL NERVOUS SYSTEM, HYDRAZINE DERIVATIVES, RESERPINE, ENZYMES, INHIBITION

Distribution Statement : APPROVED FOR PUBLIC RELEASE