Accession Number : AD0621618

Title :   DISTRIBUTION OF PROTEINASE IN CULTURES OF A SPECIES OF MICROCOCCUS IN SYNTHETIC MEDIUM,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOSCIENCES

Personal Author(s) : McDonald,I. J.

Report Date : 27 JAN 1965

Pagination or Media Count : 9

Abstract : Cultures of Micrococcus sp. (ATCC No. 407) in synthetic medium contained proteinase giving optimum casein hydrolysis at pH 5.7-6.3. The proteinase (named pH 5.7 proteinase) was found in the supernatant fluid of centrifuged cultures; the amount in the medium increased during the log phase of growth and reached a maximum just before cultures entered the maximum stationary phase. The occurrence of pH 5.7 proteinase in the external medium did not seem to be associated with cell lysis. Cells harvested at intervals during growth of cultures contained a small, nearly constant amount of pH 5.7 proteinase suggesting the enzyme is liberated by cells at about the same rate as it is formed. No evidence was obtained to suggest that cells contained inactive proenzyme or precursor. Cells contained peptidase activity and pH 7.0 proteinase (optimum casein hydrolysis at pH 7.0). These enzymes were liberated when cells were ruptured and apparently are produced by cells independently of pH 5.7 proteinase. (Author)

Descriptors :   (*MICROCOCCUS, PEPTIDE HYDROLASES), (*PEPTIDE HYDROLASES, MICROCOCCUS), CULTURE MEDIA, GROWTH(PHYSIOLOGY), PH FACTOR, ENZYMES, ENZYME PRECURSORS, CANADA

Distribution Statement : APPROVED FOR PUBLIC RELEASE