Accession Number : AD0622745

Title :   HISTOCHEMICAL STUDIES OF BROWN PIGMENTATION OBSERVED IN THE LYMPH NODES OF BOVINES AND IN THE JEJUNAL MUCOSA OF HUMANS (STUDIO ISTOCHIMICO DELLA PIGMENTAZIONE BRUNA (PIGMENTATIO FUSCA) OSSERVATA IN LINFONODI DI BOVINI E NELLA MUCOSA DIGIUNALE UMANA),

Corporate Author : BLOOD RESEARCH FOUNDATION CENTER TORTONA (ITALY)

Personal Author(s) : Ceretto,F. ; Astaldi,G. ; Penna,R.

Report Date : 1965

Pagination or Media Count : 17

Abstract : Histochemical researches on appositio slides and tissue sections demonstrated that brownish pigment previously observed by the Authors in the human jejunal mucosa and in lymph nodes from normal cattle is basophilic, acid-fast (long Ziehl-Neelsen), not metachromatic and not sudanophilic. On the contrary the pigment shows reduction sites, carboxil groups or oxy-acid; it does not demonstrate aldehydes, ketones, and proteins-end groups; it does not contains iron, bile and carotenoid-pigment, and hemofuscin. Besides, the pigment is not soluble in acids, alkali and various solvents, and may be not bleached by chlorate, H2O2 and 40% peracetic acid; it does not polarize the light and it is not fluorescent. The Authors suggest that pigment is a particular type of lipofuscin in a mittle stage of oxidation, PAS negative and acid-fast which may be classified at an intermediate level between lipofuscin sensu strictu and melanin, likewise of the pseudomelanosis pigment and considered of exogenous origin. (Author)

Descriptors :   (*HISTOLOGICAL TECHNIQUES, ORGANIC PIGMENTS), (*ORGANIC PIGMENTS, HISTOLOGICAL TECHNIQUES), LYMPHATIC SYSTEM, BOVINES, INTESTINES, MUCUS, MEMBRANES(BIOLOGY), HUMANS, TISSUES(BIOLOGY), BIOLOGICAL STAINS, ITALY

Distribution Statement : APPROVED FOR PUBLIC RELEASE