Accession Number : AD0623004

Title :   ELECTROCHEMISTRY OF ENZYMES. DETERMINATION OF PEROXIDASE AND CATALASE.

Descriptive Note : Rept. for Jan 63-Jan 64,

Corporate Author : CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD

Personal Author(s) : Guilbault,George C.

Report Date : SEP 1965

Pagination or Media Count : 19

Abstract : Two representative enzyme systems, peroxide-catalase and peroxide-peroxidase, were used in the development of an electrochemical method for their determination. An electrochemically active substrate was oxidized to two electroinactive products by proper control of the current and experimental conditions. A proton donor with nonelectroactive oxidation and reduction forms was used with peroxidase to evolve its enzymatic function. The donor was not electroactive at the potentials used. With this procedure, 0.0100 to 1.00 unit of peroxidase or catalase was determined with a relative standard deviation of 1.7%. The potentials used in the described method were studied by anodic polarographic techniques to determine their significance. (Author)

Descriptors :   (*ENZYMES, CHEMICAL ANALYSIS), (*CATALASE, ELECTROCHEMISTRY), (*PEROXIDASES, ELECTROCHEMISTRY), POLAROGRAPHIC ANALYSIS, PEROXIDES

Distribution Statement : APPROVED FOR PUBLIC RELEASE